Metabolic Enzymes of Cocaine Metabolite Benzoylecgonine
نویسندگان
چکیده
منابع مشابه
Variations in abundance of the molecular ion of the derivatized cocaine metabolite benzoylecgonine.
Benzoylecgonine (BEG) is the principal urinary metabolite of cocaine. For forensic drug testing, the presence of BEG in urine, suggested by a positive result for a screening immunoassay, must be confirmed by quantitative gas chromatographic/mass spectrometric (GC/MS) analysis, i.e., stable isotope dilution with a deuterium-labeled internal standard. GC/MS criteria for positivity also require ap...
متن کاملEvaluation of the radioimmunoassay for benzoylecgonine (a cocaine metabolite) in human urine.
The 125I-radioimmunoassay (RIA) for benzylecgonine (cocaine metabolite) in urine was evaluated by comparison with gas-liquid chromatography (GLC) and thin-layer chromatography (TLC) and the Enzyme-Multiplied Immunoassay Technique (EMIT). By RIA, a statistically significant concentration, 2 microng/liter, was observed for urinary benzoylecgonine. The coefficient of variation for the RIA was 2.58...
متن کاملDevelopment and validation of an analytical method for the simultaneous determination of cocaine and its main metabolite, benzoylecgonine, in human hair by gas chromatography/mass spectrometry.
A new, simple and rapid procedure has been developed and validated for the determination of cocaine and its main metabolite, benzoylecgonine, in human hair samples. After extraction from within the hair matrix by a mixture of methanol/hydrochloric acid (2:1) at 65 degrees C for 3 h, and sample cleanup by mixed-mode solid-phase extraction (SPE), the extracts were analyzed by gas chromatography/m...
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Carboxylesterases are important in the metabolism of cocaine, catalyzing the hydrolysis of cocaine to its two major metabolites, benzoylecgonine and ecgonine methyl ester. In the presence of ethanol, some cocaine undergoes transesterification with ethanol instead of hydrolysis with water producing the active metabolite, cocaethylene. The metabolic fate of cocaethylene is unknown, but given its ...
متن کاملDetermination of Cocaine and Benzoylecgonine in Human Plasma by Lc-ms/ms
A high-throughput Liquid chromatography/ Mass spectrometry/ Mass spectrometry (LC/MS/MS) method for simultaneous quantification of cocaine (COC) and benzoylecgonine (BZE) in human plasma was elaborated. After the protein precipitation with methanol in the plasma samples, the analytes were separated on a Zorbax SB-C18 column under isocratic conditions using a 32:68 (v/v) mixture of methanol and ...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2016
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.6b00277